The College of Education for Pure Sciences discussed a master’s thesis on comparing some plasmid contents of multi-antibiotic resistant Escherichia coli bacteria isolated from different organisms in Kirkuk Governorate, for the student Muhammad Jassim Muhammad Abdullah, under the supervision of the Prof. Dr. Najdat Bahgat Mahdi. The study aimed to isolate and diagnose Eschrichia coli bacteria from different organisms. Confirmation tests were conducted to determine the type and sex of the bacteria used in the study, and to reveal some important virulence factors in these bacteria, and to conduct sensitivity tests to different antibiotics, as the isolates were multi-resistant. The response rates of the isolates to the antibiotics used in the study varied, including those that are sensitive and others resistant, as well as isolation and neutralization of plasmids for the isolates used in the study and conducting tests to detect virulence factors before and after the neutralization process to determine the extent of the presence of virulence factors and after the neutralization process. The study concluded that most of the isolates have The ability to produce many virulence factors, including biofilm, capsule and beta-lactamase enzymes. The isolates used in the study had a clear diversity in plasmid content.