Within the framework of joint scientific cooperation between the faculties of Kirkuk University, Assistant Professor Dr. Nahan Bahaa Al-Din Jaafar and Dr. Shailan Khairallah Jabbar, who are both teaching staff in the Department of biology/College of Science at Kirkuk University, participated in the committee to discuss the master’s thesis entitled “Detection of Gene Expression.” Toxic shock syndrome toxin TSST-1 in multi-antibiotic-resistant Staphylococcus aureus bacteria isolated from urinary tract patients in the city of Kirkuk” by the student (Fatima Mahmoud Tariq Bakr) in the Department of biology / College of Education for Pure Sciences/ University of Kirkuk and under the supervision of both Assistant Professor Dr. Najdat Bahjat Mahdi and professor Dr. Aqeel Hussein Ali Al-Asi.
The two lecturers explained that the aim of the study was to isolate and diagnose Staphylococcus aureus bacteria from urinary tract patients, conduct a sensitivity test for the isolates under study towards a number of antibiotics, and reveal the ability of these isolates to produce some virulence factors such as the production of enzymes (Urease, DNase, Hemolysin). , Beta-lactamase) and biofilm formation Bioflim. Finally, the gene expression of toxic shock syndrome toxin TSST-1 was investigated for a number of isolates with multiple resistance to antibiotics using real-time PCR technology.
The study concluded that the highest prevalence of S. aureus bacteria was in females, and the genetic expression of the tsst-1 gene was stable in multiple antibiotic-resistant isolates.
After extensive scientific discussion and the student’s defense on behalf of her thesis, the committee decided to accept the thesis and award the researcher a master’s degree with distinction.