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PhD thesis at the University of Kirkuk discusses the molecular and immunological diagnosis of the parasite Cryptosporidum parvum and Giardia lambliain children with diarrhea in the city of Kirkuk with an indication of the effect of some drugs on experimentally infected mice
The thesis examined "molecular and immunological diagnosis of Cryptosporidum parvum and Giardia parasite in children with diarrhea in Kirkuk city with an indication of the effect of some drugs on experimentally infected mice".
The thesis by Enas Ihsan Rashid under the supervision of Prof. Dr. Hussein Fadel Hassan and Prof. Dr. Nima Ali Ahmed aimed to diagnose intestinal parasites (Cryptosporidium and giardia) in children visiting hospitals in the city of Kirkuk and study their prevalence. It used the PCR technology and compared it with traditional methods for diagnosing parasites. Immunological tests were conducted to determine the level of cellular kinetics in patients with diarrhea. In addition, it determined the type of parasite causing it. Furthermore, it evaluated the effect of metronidazole in the treatment of parasites in empirically infected mice.
The study concluded that most patients with diarrhoea were infected with the Cryptosporidium parasite and Giardia lamblia. The results showed significant differences in the percentage the parasite by age and sex. It also confirmed the effectiveness of the modified Ziehl-Neelsen’s Stain in diagnosing egg bags of the Cryptosporidium parasite with the superiority of PCR technology over microscopic examination for its high sensitivity. Additionally, it showed a close association between high levels of TGF-β and Caspase 3 and cases of parasite infestation. Histological analysis of the small intestine proved the effect of parasites in causing multiple histological changes, including necrosis of columnar cells lining the villi and mucosal aggregation. Moreover, there was an effect of metronidazole in different concentrations in the treatment of parasite infestation.
