حصل التدريسي في كليتنا (م.م. مصطفى محمد عمر) على قبول نشر للبحث الموسوم (Isolation and Identification of Bacillus Sp. Producing of Amylase from Different Sources in Kirkuk- Iraq) من مجلة Plant Archives

إذ هدفت الدراسة الى الحصول على عزلة محلية من بكتريا  sp. قادرة على انتاج انزيم Amlase وبفعالية عالية، تم تشخيص العزلة المنتخبة باستخدام Vitek 2 compact system و (16S rRNA gene using polymerase chain reaction (PCR

ABSTRACT

Thirty bacterial isolates belonging to Bacillus spp. obtained from different local sources in Kirkuk-Iraq were screened for their capabilities for producing Amylase. It was found that the isolates designated (5M, 8M, 9M, 12M, 13M, 17M, 18M, 24M, 27M, 30M) have higher capability for producing Amylase. All isolates subjected to secondary screening. The Isolate (13M) was found to be the highest producers with enzyme activity of (6.329U/ml). The identification tests of this isolate were carried out by studying the morphological, microscopic characteristics, and biochemical test using Vitek 2 compact system. The tests results were confirmed by identification of 16S rRNA gene using polymerase chain reaction (PCR) and its nitrogen base sequencing. The results revealed that this isolate belongs to Bacillus subtilis. Recorded in gene bank under the ACCESSION: MN744663.